However, the mechanisms for activation have remained elusive partly because of the difficulty in detecting activation-specific phosphorylation of Akt by immunoblotting in most of the JAK2-V617F-positive post-MPN sAML cell lines, including PVTL-1, PVTL-2, and HEL cells [6, 36]. This evidence concerns the gene JAK2 and myeloproliferative neoplasm.