To further illustrate the value of our novel CD133 47–10 antibody for assessing CD133 levels in tumor cells and tissue, we compared the performance of this antibody, in immunofluorescence microscopy and flow cytometry applications, to 3 commercially available anti-CD133 monoclonal antibodies that have been used for analysis of clinical specimens: clones W6B3C1, 293C3, and AC133 from Miltenyi Biotec [7, 29, 30]. This evidence concerns the gene PROM1 and neoplasm.