In the case of CD133 47–10, the immunofluorescence and immunohistochemical signals in HT29 cell pellets and human parotid tumor tissue, respectively, were blocked by peptides corresponding to the epitope-containing region in the first extracellular loop (“domain A”; Fig 3D and S3 Fig); however, the CD133 47-10 immunofluorescence signal in HT29 cells was not blocked by a peptide corresponding to the second extracellular loop (“domain B”), further demonstrating the selectivity of this reagent (S3 Fig). Here, PROM1 is linked to parotid gland neoplasm.