To examine this, we carried out fluorescent in situ hybridization (FISH) of HeLa cells infected with either Wt or Δ22 viruses and fixed at 4 h or 8 h after infection and compared the localization of five virus transcripts: ICP0 and ICP27 as representative immediate early mRNAs; TK as a representative early mRNA; and VP16 and vhs itself, which are expressed with late kinetics. Here, TKT is linked to infection.