To further examine the role WDR5 plays in cancer, the effect of WDR5 depletion (oligos #7 and #8 only) and OICR-9429 treatment on H3K4Me3, H3K4Me1, and phosphorylation of H2AX (γH2AX) was examined in HCT116, SW620, and RKO cells. This evidence concerns the gene WDR5 and cancer.