The novel findings of this study are that patients with T2D show an intact regulation of muscle gene expression and circulating levels of a panel of both well‐established (IL‐6) and novel putative myokines (IL‐15, FGF21, ANGPTL4, CHI3L1, CYR61 and CTGF) in response to acute exercise. This evidence concerns the gene FGF21 and type 2 diabetes mellitus.