We could confirm the RNAseq data in an in vivo experiment, where R7 T cells were adoptively transferred to congenic mice and analyzed in LNs 5 days after infection with Toxoplasma. CTLA4 surface expression determined by FACS in R7-II cells was highest in comparison to R7-I and R7-III CD8 T cell clones (Figure 3E). The gene discussed is CD8A; the disease is infection.