The production of TNF-α by CD4+ T cells from ND AML patients (12.21 ± 4.78%) was significantly higher as compared with that observed in healthy controls (6.78 ± 3.21%, P = 0.001) or CR AML patients (8.60 ± 4.62%, P = 0.046) (Figures 3A,B). This evidence concerns the gene TNF and acute myeloid leukemia.