In addition, autophagy was induced in the HeLa cells during the EV71 infection, and the LC3-II/LC3-I ratio gradually increased as the infection proceeded from 4 to 24 h p.i. as shown in Figure 4D. Although both the numbers of autophagosomes (Figure 4E) and LC3-II/I ratio (Figure 4F) were higher in the PML−/− cells than those in the PML+/+ cells under the mock infection, the numbers of autophagosomes and LC3-II/I ratio were elevated in PML+/+ but not in PML−/− cells following infection with EV71 (Figures 4E,F). The gene discussed is PML; the disease is infection.