TBR1 and Zika virus infectious disease: To confirm whether the structures of the cortical plate is impaired due to DCX downregulation by ZIKV infection, mock- or ZIKV-infected fetal brain were examined by detecting different cortical layer markers: Tbr1 (specific for cortex layer VI); and Ctip2 (specific for cortex layer V) at E18.5 (Figure 7C, left panels).