Western blot assessment of HMGB1 in lysates from the aortas of CKD and sham-operated mice and densitometric quantification showed no significant differences inHMGB1 expression in total lysates (Figure 1G; Figure S1); however, cytosolic HMGB1 expression was significantly higher in CKD mice than in sham mice and significantly higher in mice fed a high Pi diet than in those fed a normal diet (Figure 1H; Figure S2). This evidence concerns the gene HMGB1 and chronic kidney disease.