In addition, western blotting of lysates of the aorta and densitometric quantification showed that the siHMGB1 lentivirus s knocked down HMGB1 in the aorta, HMGB1 knockdown reversed the high Pi-induced upregulation of TLR4 and downregulation of klotho in CKD mice (Figures 3C–G; Figures S4, S5). This evidence concerns the gene HMGB1 and chronic kidney disease.