In this sense, it is known that in chronic kidney disease macrophage-derived MVs trigger the calcification of atherosclerotic plaques through high concentration of the calcium binding protein S100A9 and annexin V. In addition, after adding Ca2+/P to macrophage cell culture, phosphatidylserine translocates to MV external membrane and binds to S100A9-Annexin V complex, promoting hydroxyapatite nucleation. The gene discussed is ANXA5; the disease is chronic kidney disease.