Image analysis quantification showed that the expression of nesfatin-1 (270.67 ± 60.82, in the MS group, vs 191.33 ± 45.87, in the NH group, p = 0.029; n = 6 for each group) or TPH (167.00 ± 29.75, in the MS group, vs 120.50 ± 33.99, in the NH group, p = 0.03; n = 6 for each group) was significantly elevated in the DRN of MS rats, as shown in Fig. 3B,C. This evidence concerns the gene TPH1 and myeloid sarcoma.