However, binding affinity and STAT3/DNA binding inhibition were only obtained at high concentrations (IC50 ≈ 30 μM) Consequently, InS3-54 treatment of human breast and lung cancer cell lines results in apoptosis but also in reduced cell migration and invasion, in correlation with reduced expression of the STAT3 downstream target MMP-9. The gene discussed is STAT3; the disease is lung carcinoma.