The screen for mutations enriched after infection with Stx+ and T3SS+ EHEC identified numerous loci known to be associated with sphingolipid and glycosphingolipid biosynthesis, in particular factors required for production of the Stx receptor Gb3, as well as two loci (TM9SF2 and LAPTM4A) with largely undefined cellular roles that were also required for toxin binding. Here, TM9SF2 is linked to infection.