To determine and compare the effects of the loss of PTPRS on the expression of these genes in CRC cell lines, we compared HCT116 (KRAS G13D/+) to HCT116 (−/+) in paired cell lines, with and without CRISPR KO of PTPRS. In addition, we also investigated these same ERK regulated genes in KM12L4A (wild-type KRAS) and SW620 (mutant KRAS) PTPRS CRISPR KO cell lines. Here, MAPK1 is linked to colorectal carcinoma.