To confirm a potential regulatory role of PTPRS in RAS pathway activation, we inhibited PTPRS activity in vivo in CRC cell lines containing both mutation-activated and wild-type KRAS (i.e. HCT116 (KRAS G13D), SW620 (KRAS G12V) and KM12L4A (WT KRAS)). The gene discussed is PTPRS; the disease is colorectal carcinoma.