olig2 [tumor 11.2-fold, Benjamini-Hochberg adjusted P-value (Padj)<0.00001; mutant 1.1-fold, Padj= 0.71904] and ascl1b (tumor 18-fold, Padj<0.00001; mutant 0.9-fold, Padj=0.69777) levels were confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Table S8) in rb1 tumor tissue compared with normal adult brain (Fig. 4C), and in the 5 dpf rb1/rb1 mutant larvae compared with wild type (Fig. 4D). The gene discussed is OLIG2; the disease is neoplasm.