What’s more, by knocking down the GOLPH3 expression in HCC cells, the expression of p-mTOR (Ser2448), p-Akt (Ser473) and p-S6 K1 (Thr389) were remarkably decreased in the HCC cell lines and in the xenograft tumor model (Fig. 5a and Fig. 5b). This evidence concerns the gene AKT1 and hepatocellular carcinoma.