And western blotting analysis showed that phosphorylated mTOR, AKT and S6 K1 level were dramatically higher in the HCC cell lines compared with those in the control group (Fig. 4b).What’s more, knockdown GOLPH3 down-regulated the phosphorylation of mTOR, AKT and S6 K1 in MHCC97L and MHCC97H (Fig. 5a). The gene discussed is AKT1; the disease is hepatocellular carcinoma.