The five TLR proteins were identified in tumor cells, in both GBM cell lineages with mutational status consistent for the mesenchymal subtype (U87MG and A172): RB transcriptional corepressor (RB1) missense mutation in A172, and neurofibromin (NF1) missense and deletion mutation in U87MG [37]. The gene discussed is RB1; the disease is glioblastoma.