Sorted NKp46+CD3− cells (98.5% purity) were expanded with IL-2 and used in ATCC against P815 (E:T ratio 2.5:1), in crystal violet assay with autologous TAF or in cytotoxicity assay (E:T ratio 10:1) against Caco2 or HCT15 CRC cell lines. The gene discussed is IL2; the disease is colorectal carcinoma.