NCR1 and colorectal carcinoma: In 45 CRC specimens, cell suspensions obtained from the tumor (K) or the non-tumor (N) areas, were cultured 12 h in flat-bottom plates to remove adherent cells; then, non-adherent cells were stained with anti-CD45 (leukocyte marker), anti-CD3 (T cell marker), and anti-NKp46 (NK cell marker) mAbs, followed by FACS analysis.