This fact was also observed during the course of T. cruzi infection in experimental infections of CD1 mice with the Brazil strain of parasites, showing that the p53 protein is upregulated in the liver 45 days post-infection, which might result in an elevated secretion of liver EVs into the bloodstream of patients with acute Chagas disease (Bouzahzah et al., 2006). Here, TP53 is linked to infection.