Leptin (200 nM) increased the expression of TGF-β1 in KCs and sinusoidal endothelial cells, and connective tissue growth factor in KCs (Ikejima et al., 2002), being KCs-HSCs cross-talk proposed for liver fibrosis (Wang et al., 2009). This evidence concerns the gene TGFB1 and Hepatic fibrosis.