CRLF2 and precursor B-cell acute lymphoblastic leukemia: Jak2 knockdown in Eμ-Crlf2/Jak2R683G cells abrogated pStat5Y694 and pS6S240/244 (Fig. 3B), and RNA-seq analysis of Eμ-Crlf2/Jak2R683G B-ALL with Jak2 knockdown identified gene signatures that were significantly enriched in JAK2-depleted MHH-CALL4 cells, indicating that a similar pattern of transcriptional change is induced upon RNAi-mediated JAK2 depletion across the two model systems (false discovery rate [FDR] < 0.05) (Fig. 3C).