Using a combination of in silico and in vitro approaches to find and validate putative targets, we found that MIR194 could repress GRHL3 likely through its conserved binding site in the 3′ UTR of GRHL3. GRHL3 positively regulates the tumor suppressor PTEN, whereas GRHL3 knockout results in squamous cell carcinoma development in vivo associated with activation of phosphatidylinositol-3-kinase signaling.41 This evidence concerns the gene GRHL3 and squamous cell carcinoma.