These tests showed that LIR1 KO metacyclics were as viable as WT metacyclics prior to macrophage infection (Fig 6b), and there were also no differences in the ability of WT or KO parasites to induce formation of the enlarged parasitophorous vacuoles (PV) that are typical of L. amazonensis [37] in macrophages (Fig 6c). The gene discussed is LILRB1; the disease is infection.