Treatment of 28 DIV 3xTg-AD slice cultures with 100 nM NAPVSIPQ for 24 h significantly reduced tau phosphorylation at the Thr231 epitope, but did not alter the total amount of tau when compared to control cultures16, thus we find that equivalent treatment of 3xTg-AD organotypic brain slice cultures recapitulates previousin vivo findings conducted in 3xTg-AD mice38–40. The gene discussed is MAPT; the disease is Alzheimer disease.