Among them, three sgRNAs introduced mutations reproducing that seen in patients (http://www.uniprot.org/docs/humsavar): sgAr-1 and sgAr-15 caused S683G and I878T at the Ar locus linked to AIS, while sgHoxd13 caused Q321R at the Hoxd13 locus associated with Syndactyly diseases (Supplementary Fig. 1a, b). This evidence concerns the gene HOXD13 and syndactyly.