AR and androgen insensitivity syndrome: Among them, three sgRNAs introduced mutations reproducing that seen in patients (http://www.uniprot.org/docs/humsavar): sgAr-1 and sgAr-15 caused S683G and I878T at the Ar locus linked to AIS, while sgHoxd13 caused Q321R at the Hoxd13 locus associated with Syndactyly diseases (Supplementary Fig. 1a, b).