Three subsets of immune cells were used in standard cytotoxicity assays as effectors: CD19CAR-Jurkat cells sorted to 100% purity (which kill through CD19 recognition), activated (3000 IU/mL IL-2 + 50 ng/mL αCD3 mAb) Jurkat and lymphokine activated killer (LAK) cells (both of which kill indiscriminately and independently of CD19 or MHC) efficiently killed NHL lines, albeit to varying degrees (Figure 2A–C). This evidence concerns the gene CD19 and non-Hodgkin lymphoma.