Our objective was to determine the prevalence of PIK3CA copy number gain and correlate this with the activity status of PI3K-AKT-mTOR pathway in different disease states of penile cancer via utilisation of a large cohort of both pre-cancerous penile intraepithelial neoplasia (PeIN) and invasive PSCC cases. This evidence concerns the gene AKT1 and squamous cell carcinoma of penis.