The application of >500×cov and >1% AF mutation criteria may increase detectable mutations due to the increased NGS diagnostic accuracy, that may detect tumoral clonal heterogeneity: KRAS2–4 mut were prevalently detected at >1000×cov/>5% AF (50.8%), while NRAS2–4 mut (16.4%) were frequently detected at 500–1000xcov and <5%AF (6%), and BRAF15 mut (7.5%) were all detected at 500–1000×cov and <5%AF. This evidence concerns the gene KRAS and atrial fibrillation.