The results of the microarrays performed in the present study also revealed that the gene expression of important factors in cellular movement, such as MMP1, MMP12, and TIMP4, metalloproteinase inhibitor, was changed by the knockdown of AE2, suggesting that AE2 regulates the tumor behavior of ESCC via this pathway. The gene discussed is TIMP4; the disease is esophageal squamous cell carcinoma.