To investigate the chain of events leading to the loss of cell viability, we initially stained the B-CLL cells with TO-PRO-3 iodide, which binds with high affinity to double-strand nucleic acids but does not enter intact plasma membrane, and Annexin-V to determine the lipid phosphatidylserine flip from the inner to the outer leaflet of the plasma membrane. Here, ANXA5 is linked to B-cell chronic lymphocytic leukemia.