Hydrodynamic injection of siRNA or shRNA naked plasmid could inhibit the genes expression in the liver (McCaffrey et al., 2002; Song et al., 2003), Abe et al. (2016) also has recently found that by mediating MMP-13 expression via hydrodynamic injection could effectively inhibit liver fibrosis in rats. Here, MMP13 is linked to Hepatic fibrosis.