In this study, we sequenced 75 RP and 10 Usher small families by direct Sanger sequencing of the exons of USH2A. We identified six novel mutations in the USH2A gene causing nonsyndromic RP or Usher syndrome in a Chinese population; this finding provides a theoretical basis for follow-up research and treatment of this disease. Here, USH2A is linked to retinitis pigmentosa 1.