In general, the ICG clearance test has certain limitations, as constant hemodynamic conditions (stable liver perfusion rate and hepatic blood flow) are required for liver function analysis40–42 and, in cases of cholestasis and hyperbilirubinemia, carrier competition of bilirubin and ICG at the OATP1 transporter might occur43,44. Here, SLCO1A2 is linked to cholestasis.