TNF and Familial prostate cancer: To understand the mechanism underlying the apoptosis induced by ANO1 inhibition, we performed the gene profiling analysis in prostate cancer PC-3 cells and found that ANO1 downregulation caused an increased expression of death receptor signaling molecules, such as TNF-α, TNFSF10 (TRAIL), and CASP7 (Supplementary Table 1 and Supplementary Figure 4).