To further test this hypothesis, in addition to the HFD-induced T2D model, we examined the effect of Becn1F121A-mediated autophagy hyperactivation in db/db mice, a genetic model of obesity and T2D lacking the functional leptin receptor (Chen et al., 1996; King, 2012). This evidence concerns the gene LEPR and obesity due to melanocortin 4 receptor deficiency.