To represent the biology of schwannoma formation, we employed a matched pair of human fetal Schwann cell (SC) lines, (HS11, wild-type) and (HS01, NF2-shRNA suppressed with ~3% merlin protein expression by Western blotting and ~7% RNA expression by RNA sequencing versus wild-type) as well as a series of four mouse merlin-wildtype and -deficient SC and a merlin-deficient schwannoma lineS [13,14] All control AC and SC lines expressed a merlin band at ~70 kDa by Western blot, whereas human and mouse cell lines with NF2 or Nf2 inactivation, respectively, failed to exhibit merlin protein (S1 Fig). The gene discussed is NF2; the disease is schwannoma.