On the contrary, another study indicated that after cerebral ischemia lasting 30 min, white matter damage was similar between animals treated with the combination of IGF-1 (3 μg, ventricularly) and hypothermia (30-33°C) from 5.5 h after ischemia until 72 h after ischemia, and animals treated with hypothermia alone, based on immunohistochemistry and numbers of caspase 3-positive cells [121]. This evidence concerns the gene CASP3 and ischemia.