The percentage of CD3+CD45RA- T cells (Fig. 6A and 6F, p<0.05 by two-way ANOVA), including CD3+CD4+ Th cells (Fig. 6B and 6G, p<0.05 by two-way ANOVA) and CD3+CD8+ Tc cells (Fig. 6C and H, p<0.05 by two-way ANOVA) among total lymphocytes after stroke significantly decreased in rats with splenectomy. This evidence concerns the gene CD4 and Stroke.