Here, we use oxygen-glucose deprivation/reperfusion (OGD/R) and a middle cerebral artery occlusion (MCAO) mice model to mimic ischemia/reperfusion injury in vitro and in vivo to detect the role of iASPP in cerebral ischemia treated with local mild therapeutic hypothermia. The gene discussed is PPP1R13L; the disease is Cerebral ischemia.