TNF and infection: Since it has been described that infected mice previously vaccinated with a recombinant adenovirus expressing ASP-2 display higher levels of polyfunctional CD8+ T cells, compared to non-vaccinated infected mice (18), we also analyzed here the percentages and absolute numbers of CD8+ T cells producing simultaneously TNF and IFN-γ, or TNF and GzB, or GzB and IFN-γ in the four experimental groups (naïve controls, infected-only, fd-WT + infection, and fd-PA8 + infection).