While ABCG8-deficiency did not affect basal plasma cholesterol, the LDLR-deficient dams displayed marked hypercholesterolaemia (5.2-fold change, p < 0.01, Fig. 1a), mostly due to increased cholesterol levels in LDL and VLDL (Fig. 1b–d). This evidence concerns the gene ABCG8 and Hypercholesterolemia.