Knockdown efficiency was examined by qtPCR and western blot, our results showed that IL-6 siRNA reduced IL-6 expression by over 90% during the first 72 hours in breast cancer cells (Fig. 5a–c), whiles in adipocytes IL-6 siRNA reduced IL-6 expression by about 50% with 24 hours (Fig. 5d,e) but expression of IL-6 increased with 48 hours. This evidence concerns the gene IL6 and breast cancer.