Previous genomic characterization of this cell line showed mutation in Kras and lack of mutations in Apc, Tp53, Braf, Pold1, and MMR genes Mlh1, Msh2, Msh6, and Pms2, indicating CT26 cell line represents a MMR-proficient colorectal cancer type6,7. Here, POLD1 is linked to colorectal cancer.