By luciferase reporter assay, we confirmed that miR-216a-5p could directly bind to HOTTIP, and co-transfection luciferase reporter assay revealed that HOTTIP could reverse the inhibited reporter plasmid luciferase activity, indicating that HOTTIP can act as endogenous ‘sponge’ for miR-216a in PCa cells. This evidence concerns the gene HOTTIP and posterior cortical atrophy.