We further used qRT-PCR to demonstrate the expression of HOTTIP in a panel of human PCa cell lines, and we found that its levels in four human PCa cell lines DU145, PC3, LNCaP, and 22Rv1, were higher than the levels in the RWPE1 human normal prostate epithelial cell line (P<0.01, Figure 1B). The gene discussed is HOTTIP; the disease is posterior cortical atrophy.