Of note, although PK M2 and LDH enzyme activities in individual patients were changed (normoxia compared to hypoxia), the protein expression of these and other glycolytic enzymes was unchanged (normoxia compared to hypoxia) as shown in western blot analysis of PK M2, LDH and GAPDH in 4 CLL study patient samples classified as MS HR and 4 classified as MS LR (Supplementary Fig. S2). The gene discussed is GAPDH; the disease is B-cell chronic lymphocytic leukemia.