HDGFL2 and malaria: This method of defining malaria “positivity” also presents a problem from a converse perspective: if a treated P. falciparum infection generated a negative HRP2 band and a positive pan-pLDH band (due, perhaps, to circulating mature gametocytes not cleared by anti-malarial medication and producing pLDH), then the RDT may be misreported as a malaria infection by a species other than P. falciparum [39].