In the end, we measured the ECAR in ERK2E322K/KL overexpression colon cancer cells, and demonstrated that ERK2E322K overexpression increased the ECAR value in KL overexpressed cells (P < 0.05), suggesting the role of ERK on KL mediated glycolysis regulation (Fig. 5g). This evidence concerns the gene MAPK1 and malignant colon neoplasm.