Despite proper standardization of IL-10 staining in positive control samples from human intestine which were run in parallel, no IL-10 staining was seen in leprosy T1R or T2R or reaction-free lesions, while adequate staining for TGF-β, IL-17 and IFN-γ was achieved in leprosy lesions. This evidence concerns the gene TGFB1 and leprosy.