A parallel analysis of CD3+ T cells revealed that CXCR3 expression was reduced in four of the eight areas identified by SPADE (Fig. 4a, b) in individuals with long-standing diabetes vs healthy donors (areas 2, 4, 5 and 6: p < 0.005 in Study A, p < 0.005 in Study B; Fig. 4c–f). The gene discussed is CXCR3; the disease is diabetes mellitus.